There is no obvious boundary between the colonies growing in a chain shape. As a colony count, if there are several chains from different sources, each chain should be counted as one colony.
If the plate-shaped colonies are less than half of the plate, and the other half is evenly distributed, the number of colonies on half a plate can be multiplied by 2 to represent the number of colonies on the whole plate. Selection of plate colony count
Select a plate with a number of colonies between 30 and 300 (SN standard requires 25 to 250 colonies). If there are two dilutions between 30 and 300, the ratio is less than or equal to 2 and the average value is greater than 2 Then its smaller number.
If both are greater than 300, the average number of colonies at the highest dilution is multiplied by the dilution factor to report. ? If both are less than 30, report the average number of colonies at the lowest dilution times the dilution factor.
If the number of colonies is more than 300, and some is less than 30, not between 30 and 300, the average colony number closest to 300 or 30 is multiplied by the dilution factor to report.
If all dilutions are aseptically grown, they should be reported as less than 1 times the lowest dilution multiple.
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