Rabbit Anti-IL-2 antibody introduction

Catalog Number: Rs- 1191R

Rabbit Anti-IL-2 antibody introduction

Quantity size: 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent)

Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7 ). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. It produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Belongs to the IL-2 family.

Specificity:

· Anti-IL-2 is a rabbit monoclonal antibody unconjugated

· Recognizes Interleukin-2 of mouse rat, hu

· Epitope mapping recombined IL-2 of mouse rat, hu

· Use for western blotting, elisa, immunoprecipitation

and immunohistochemistry

· Protein G affinity chromatography purification, purity:> 95%

· Isotype: IgG

· Mol wt: 17kDa

Rabbit Anti-IL-2 antibody introduction

Application:

· Western blotting 1: 100-500

· Immunohistochemistry 1: 100-500

· ELISA 1: 500-1000

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze / thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.