Shanghai Yiji Industrial Co., Ltd. is a high-tech enterprise integrating R & D, sales, and technical services. It sells and produces a variety of medical research reagents, mainly engaged in ELISA kits, cells, serum, antibodies, biological reagents, culture media , And other products, please call for details.
English name: Taq plus DNA Polymerase
Other names: Taq plus DNA polymerase
Level: BR
Purity: ≥99.0%
Concentration: 5u / ul
Vitality definition: the amount of enzyme required to infiltrate 10 nmol of isotope-labeled dNTPs into DE81 paper insolubles within 72 minutes at 72 ° C
10 × Taq Plus Buffer: 200mM Tris-HCL (PH8.8), 100mM KC1, 100mM (NH4) 2SO4, 20mM Mg2SO4, 1% Triton X-100, 1mg / ml BSA
Storage buffer: 20mM Tris-HCL (PH8.0), 0.1mM EDTA, 1mM DTT, 0.5% (V / V) Tween 20, 0.5% (v / v) Nonidet P40 and 50% (v / v) :liquid. Taq plus DNA Polymerase is a DNA polymerase prepared by a special process. It is a mixed enzyme of ordinary Taq enzyme mixed with a certain proportion of pfu enzyme. The purity of each enzyme is above 95%. After testing, there is no pollution of nuclease and foreign DNA. With Taq enzyme super-amplifying ability, at the same time due to the presence of pfu enzyme can also partially correct errors in DNA amplification, fidelity is about 3 times that of Taq enzyme.
Uses: biochemical research. After testing the activity without exogenous nuclease, using lambda DNA as a template, it can amplify 2kb DNA fragments. Can be used for conventional PCR amplification, high-fidelity amplification and expression of genes, and site-directed mutation storage of genes: -20 ℃, shelf life 1 year
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