Application of flow cytometry injection (flow cytometry)

â‘  Cell biology: quantitative analysis of the cell cycle and sorting of cells of different cell cycle phases; analysis of the relationship between biological macromolecules such as DNA, RNA, antigens, oncogene expression products and other cell proliferation cycles, and karyotype analysis And can purify X or Y chromosome.

â‘¡Oncology: The determination of DNA ploidy content is a specific indicator for identifying benign and malignant tumors. In recent years, DNA ploidy assay technology has been applied to detect various solid tumor cells such as leukemia, lymphoma, lung cancer, bladder cancer, and prostate cancer. Use the monoclonal antibody technology to remove tumor cells from the blood.

â‘¢Immunology: study the relationship between cell cycle or DNA ploidy and cell surface receptor and antigen expression; type and purify immune active cells; analyze the relationship between lymphocyte subsets and diseases; diagnosis of immunodeficiency diseases such as AIDS Immunological monitoring after organ transplantation, etc.

â‘£Hematology: classification and typing of blood cells, differentiation of hematopoietic cells, quantitative analysis of various enzymes in blood cells, such as peroxidase, non-specific esterase, etc .; leukemia cells can be studied with NBT and DNA double staining The relationship between differentiation and maturation and changes in the cell proliferation cycle. Detect Rh (+) or anti-D antigen positive cells in the maternal blood to understand whether the fetus may have severe hemolysis due to Rh blood group incompatibility; detection of circulating immune complexes in the blood can diagnose autoimmunity Sexual diseases, such as lupus erythematosus.