The company specializes in supplying Elisa kits, quality assurance, price concessions, and can provide free testing services, please call to consult!
This kit can only be used for scientific research, not for medical diagnosis.
Human interleukin 4 (IL-4) quantitative detection kit (ELISA)
user's Guide
[Human interleukin 4 (IL-4) Elisa kit kit name]
Human interleukin 4 (IL-4) quantitative detection kit (ELISA)
[Human interleukin 4 (IL-4) Elisa kit kit uses]
Quantitatively detect the content of interleukin 4 (IL-4) in human serum, plasma and related liquid samples.
[Human interleukin 4 (IL-4) Elisa kit detection principle]
This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated human interleukin 4 (IL-4) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components, and then add the enzyme label After incubating the working solution for a sufficient period of time, wash to remove unbound components. Substrates A and B are added in sequence. The substrate (TMB) is converted into a blue product catalyzed by horseradish peroxidase (HRP) and turns yellow under the action of an acid. The shade of the color and the human interleukin in the sample The concentration of 4 (IL-4) is positively correlated. The OD value is measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of human interleukin 4 (IL-4) in the sample is calculated.
[Composition of Human Interleukin 4 (IL-4) Elisa Kit Kit]
1
Enzyme coated plate
12 holes × 8
7
Developer A liquid
6mL
2
Standard
0.3mL × 6 tubes
8
Developer B liquid
6mL
3
20 times concentrated washing liquid
25mL
9
Stop solution
6mL
4
Sample diluent
6mL
10
Instructions
1 serving
5
Special diluent
6mL
11
Sealing film
2 sheets
6
Enzyme reagent
6mL
12
sealed bag
1
Remarks: The concentration of standard products (No. 1 → No. 6) is: 48, 24, 12, 6, 3, 1.5 ng / L.
[Human Interleukin 4 (IL-4) Elisa kit required but not provided reagents and equipment]
1. 37 ℃ thermostat
2. Standard specification microplate reader
3. Precision pipettes and disposable tips
4. Distilled water
5. Disposable test tubes
6. Absorbent paper
[Human Interleukin 4 (IL-4) Elisa kit operating steps]
1. Preparation: Remove the reagent kit from the refrigerator and re-equilibrate at room temperature for 30 minutes.
2. Mixing solution: dilute the 20-fold concentrated washing solution with distilled water to the original one.
3. Add standard products and samples to be tested: take a sufficient number of enzyme-coated plates and fix them to the frame. Set up standard wells, sample wells to be tested and blank control wells, record the positions of each well in the standard wells. Add 50μL of standard product; first add 10μL of sample to be tested, and then add 40μL of sample diluent (that is, the sample is diluted 5 times); blank control well is not added.
4. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes.
5. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each hole with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, repeat the washing 4 times (you can also use the washing machine to press Instructions for washing the board).
6. Add enzyme-labeled working solution: add 50μL of enzyme-labeled working solution to each well, without adding blank control wells.
7. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes.
8. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each hole with washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the plate washing 4 times (you can also use the washing machine to press Instructions for washing the board).
9. Color development: add 50μL of developer A solution to each well, and then add 50μL of developer B solution, mix for 30s with a plate mixer (or gently shake for 30s by hand), and avoid color development at 37 ℃ for 15min .
10. Termination: Remove the enzyme labeling plate and add 50μL of stop solution to each well to stop the reaction (the color changes from blue to yellow).
11. Determination: Zero the blank holes, and within 15 minutes after termination, measure the absorbance (OD value) of each well with a wavelength of 450 nm.
12. Calculation: According to the concentration of the standard product and the corresponding OD value, calculate the linear regression equation of the standard curve, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. You can also use various application software to Calculation. The final concentration is the actual measured concentration times the dilution factor.
[Human Interleukin 4 (IL-4) Elisa kit sample requirements]
1. The sample cannot contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP).
2. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided.
3. The sample should be fully centrifuged, without hemolysis and particles.
[Precautions for Human Interleukin 4 (IL-4) Elisa Kit]
1. The experiment is carried out in strict accordance with the instructions, and the result of the experiment must be determined by the reading of the microplate reader.
2. If the enzyme-labeled coated board is not used up after opening, it should be put into a sealed bag and added with desiccant immediately.
3. It is recommended that all standards, samples and blank controls be tested in duplicate, and the average value is taken to reduce the experimental error.
4. Keep in mind that the sample has been diluted 5 times. The calculation result is multiplied by 5 to obtain the actual concentration of the sample.
5. The quantitative range of this kit is 1.5-48ng / L. If it exceeds this range, it is calculated from the extension of the standard curve. ), Multiplied by the total dilution factor is the final concentration of the sample.
6. If the color is too light, the substrate incubation time can be extended properly.
7. In order to avoid cross-contamination, the tips, samples and blank controls should be replaced with a new one for each addition; the common components such as enzyme working solution, sample diluent and substrate should be cantilevered, and they should not touch the microwells. ; Do not reuse the sealing film.
8. The kits are used within the warranty period, and different batches of reagents should not be mixed.
9. Substrate B is sensitive to light and avoid prolonged exposure to light.
[Human Interleukin 4 (IL-4) Elisa kit operating procedures summary]
Prepare reagents, samples and standards
Add prepared samples and standards, and react at 37 ℃ for 30 minutes
Wash the plate 4 times, add the enzyme reagent, and react at 37 ℃ for 30 minutes
Wash the plate 4 times, add color developing solutions A and B, and develop at 37 ℃ for 15 minutes
Add stop solution
Read OD value within 15 minutes
Calculation
[Human Interleukin 4 (IL-4) Elisa kit detection range]
1.5ng / L-48ng / L
[Human Interleukin 4 (IL-4) Elisa Kit Specifications]
96 servings / box
[Storage of Human Interleukin 4 (IL-4) Elisa Kit]
Store at 2-8 ℃, protected from light and moisture.
[Human interleukin 4 (IL-4) Elisa kit expiration date]
6 months
White Primer Moulded Door Moulded Panel doors
white primer molded panel door is an interior door that features a molded panel design and comes pre-primed with a white primer coat. These doors offer a combination of style, affordability, and ease of customization. Here are some key features and benefits of white primer molded panel doors:
-
Molded panel design: White primer molded panel doors have a molded construction that creates raised or recessed panel designs on the surface. These panels add depth and visual interest to the door, mimicking the appearance of traditional solid wood doors.
-
Pre-primed with white primer: The doors come with a white primer coat already applied to the surface. The primer serves as a base layer for paint, making it easier to achieve a smooth, finished look when painting the door in the desired color.
-
Style versatility: White primer molded panel doors are available in various panel designs, such as 2-panel, 3-panel, 4-panel, or 6-panel configurations. This allows homeowners to choose a style that complements their interior decor and personal preferences.
-
Affordability: Compared to solid wood doors, white primer molded panel doors are generally more affordable. They provide a cost-effective option for homeowners who desire the look of a traditional paneled door without the higher price tag.
-
Easy customization: The white primer coat provides a blank canvas for customization. Homeowners can easily paint the door in any color or finish of their choice to match their interior design scheme. The primer layer helps the paint adhere properly and enhances the durability of the finish.
-
Low maintenance: White primer molded panel doors typically require minimal maintenance. The painted surface is easy to clean with mild soap and water to keep it looking fresh.
-
Consistency and stability: Molded panel doors are manufactured using engineered wood cores or composite materials that provide stability and reduce the likelihood of warping or twisting. This ensures that the doors maintain their shape and functionality over time.
It's important to note that white primer molded panel doors are intended for interior use only and are not suitable for exterior applications. They are commonly used for bedrooms, living rooms, closets, and other areas within the home.
White Primered Moulded Doors,Interior White Primer Door,White Primer HDF Door,Moulded Interior Doors,Moulded Panel doors,Moulded Skin Doors
Ningbo Greenly Industy Co.,LTD. , https://www.chinagreenly.com