Classification and access rules for biological reagents

Classification of biological reagents

China's reagent specifications are basically divided into purity (how much impurity content), a total of high purity, spectral purity, benchmark, spectroscopic pure, excellent grade pure, analytical and chemical purity. The state and the competent authorities promulgate three main types of quality indicators: pure, graded pure and chemically pure.

(1) (GR:Guaranteed reagent), also known as primary or guaranteed reagent, 99.8%, this reagent has the highest purity and the lowest impurity content, suitable for important and precise analytical work and scientific research work, using green bottles sign.

(2) Analytical purity (AR), also known as secondary reagent, with high purity, 99.7%, slightly below the pure grade, suitable for important analysis and general research work, using red bottle label.

(3) Chemically pure (CP), also known as tertiary reagent, ≥ 99.5%, the purity and analytical purity are quite different, suitable for industrial and mining, general analysis of schools. Use a blue (dark blue) label. ELISA kit

(4) Experimental reagent (LR: Laboratory reagent), also known as a four-stage reagent.

In addition to the above four levels, ELISA kits are currently available on the market:

(1) Reference Reagent (PT: Primary Reagent): It is used exclusively as a reference material, and a standard solution can be directly prepared.

(2) Spectral pure reagent (SP: Spectrum pure): indicates that the spectrum is pure (because the organic matter is not displayed on the spectrum, the main component may not be more than 99.9%, and must be used when using it, especially when used as a reference. Perform calibration.)

(3) High-purity reagent: The purity is much higher than that of the pure grade reagent (≥ 99.99%).

Reagent access rules

The solid powder reagent can be taken with a clean horn spoon. To take a certain amount of solids, place the solids on paper or on a watch glass and weigh on a scale. For accurate weighing, the weighing bottle is weighed on the balance. The liquid reagent is usually measured by a measuring cylinder. The volume of the measuring cylinder is 5 mL, 10 mL, 50 mL, 500 mL, etc., and the liquid to be measured is injected into the measuring cylinder during use, so that the line of sight and the lowest point of the liquid concave surface in the measuring cylinder are kept horizontal, and then read. The scale on the measuring cylinder is the volume of the liquid.

If a small amount of liquid reagent is needed, it can be taken with a dropper. When taking it, care should be taken not to touch or insert the dropper onto or into the wall of the receiving container.

In order to achieve accurate experimental results, the following rules should be followed when taking reagents to ensure that the reagents are not contaminated and degraded:

(1) The reagent cannot be in contact with the hand.

(2) To use a clean medicine spoon, a measuring cylinder or a dropper to take reagents, it is absolutely not allowed to use the same tool at the same time and continuously use a variety of reagents. After taking one reagent, the tool should be washed (the spoon should be dried) before another reagent can be used.

(3) After the reagent is taken, be sure to close the stopper. Do not put the wrong bottle cap and the dropper. Do not allow the crown to be worn. After use, put the bottle back to the original position.

(4) The removed reagent can no longer be returned to the original reagent bottle.

In addition, the use of reagents should be based on the spirit of saving, as little as possible, so that it is easy to operate and carefully observe the phenomenon, but also get better experimental results.

Item number

product name

CAS

specification

package

Price¥

C014113

Lactose broth

/

/

250g

125

D16-1025943

Corn flour agar

/

BR

250g

189.75

D16-1005369

Lactose peptone broth

/

/

250g

113.85

D16-1024734

Antibiotic Shaji agar medium

/

BR

250g

Inquiry

C84-3053

Tryptone

73049-73-7

Tryptone, BR

100g

418